| 质粒名称: | pGL3 BRE Luciferase |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 45126 |
| 存储实验室: | Martine Roussel, Peter ten Dijke |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3-Basic |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 4818 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Id1-BRE |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pGL3 BRE Luciferase was a gift from Martine Roussel & Peter ten Dijke (Addgene plasmid # 45126 ; http://n2t.net/addgene:45126 ; RRID:Addgene_45126) |
| 参考文献部分: | Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter. Korchynskyi O, ten Dijke P. J Biol Chem. 2002 Feb 15;277(7):4883-91. Epub 2001 Nov 29. 10.1074/jbc.M111023200 PubMed 11729207 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL3 BRE Luciferase 4942 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGL3 BRE Luciferase
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4942)
AUTHORS Korchynskyi O, ten Dijke P
TITLE Identification and functional characterization of distinct
critically important bone morphogenetic protein-specific response
elements in the Id1 promoter.
JOURNAL J Biol Chem. 2002 Feb 15;277(7):4883-91. Epub 2001 Nov 29.
PUBMED 11729207
REFERENCE 2 (bases 1 to 4942)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4942
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 155..164
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 161..1813
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(227..245)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 1854..1975
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1865..1884)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1919..1938
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2223..2240)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2394..2982)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2474..2493)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3153..4013)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3776..3795
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4014..4118)
/gene="bla"
/label=AmpR promoter
rep_origin 4145..4600
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4232..4251)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4442..4463
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4731..4779
/note="synthetic polyadenylation signal"
misc_feature 4793..4884
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4833..4852
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ctgtcaggct ggcgccgcgg cgccagcctg acagcccgtc ctggcgtcta acggtctgag
61 ctagcccggg ctcgagatct gggctataaa agggggtggg ggcgcgttcg tcctcactct
121 cttccaagct tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat
181 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
241 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
301 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
361 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
421 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
481 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
541 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
601 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
661 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
721 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg
781 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt
841 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt
901 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag
961 atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct
1021 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc
1081 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt
1141 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc
1201 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc
1261 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag
1321 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc
1381 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga
1441 cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta
1501 tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc
1561 aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt
1621 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac
1681 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac
1741 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa
1801 gatcgccgtg taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
1861 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
1921 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
1981 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
2041 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga
2101 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
2161 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
2221 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
2281 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
2341 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
2401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
2461 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
2521 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
2581 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
2641 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
2701 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
2761 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
2821 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
2881 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
2941 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
3001 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
3061 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
3121 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
3181 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
3241 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
3301 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
3361 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
3421 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
3481 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
3541 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
3601 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
3661 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
3721 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
3781 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
3841 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
3901 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
3961 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
4021 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
4081 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
4141 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
4201 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
4261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
4321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
4381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
4441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
4501 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
4561 tttaacgcga attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg
4621 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
4681 ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat
4741 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
4801 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
4861 gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcttacgcg tgctagctca
4921 gaccgttaga cgccaggacg gg
//
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